나노 수산화인회석이 첨가된 구강양치액의 법랑질 재광화 효과
Remineralization effect of mouthrinse containing nano-hydroxyapatite by pH-cycling model
김민영, 권호근, 김백일,
소속 상세정보
김민영 ( Kim Min-Young ) - 연세대학교 치과대학 예방치과학교실, 구강과학연구소
권호근 ( Kwon Ho-Keun ) - 연세대학교 치과대학 예방치과학교실, 구강과학연구소, 구강악안면경조직재생센터
김백일 ( Kim Baek-Il ) - 연세대학교 치과대학 예방치과학교실, 구강과학연구소
KMID : 0355420070310020156
Abstract
Objectives: The aim of this study was to evaluate the combined effects of nano-HAp (nano sized hydroxyapatite) and sodium fluoride mouthrinse (NaF) on early casies lesion in human enamel using pH-cycling model for 14 days.
Methods: Enamel specimens which had 25-45 Viclters Hardness Number (VHN) were selected from artificial demineralization for 48 hours. Eight groups of treatment solutions (O%, 1%, 5%, 10% nano- HAp in distilled water and same concentrations of nano-HAp in 0.05% NaF solution) were incubated in pH-cycling model. After pH cycling model, each specimen was evaluated by VHN. All specimens were processed for Confocal Laser Scanning Microscopy (CLSM) to investigate caries lesion. Some of specimens which were treated for 14 days were evaluated surface mineral composition by EPMA. Statistical significances were identified by one-way ANOVA followed Duncan’ s studentized range test
for variable at 0.05 significant level.
Results: The results showed that the degree of remineralization revealed by VHN values was higher in NaF groups than groups of distilled water. The VHNs of remineralized enamels for 7 days were higher than 14 days treatment. In addition, the remineralization was significantly influenced as concentration of nano-HAp was elevated and these were more affected in NaF groups than distilled water groups (p ( 0.05). These effects were confirmed by CLSM and EPMA, which exhibited nano-sized particles attached to enamel in nano-HAp treated groups. Therefore, nano-HAP might play a synergistic role for remineralization with fluoride mouthrinse. It will be required to investigate the optimal condition of
nano-HAp and NaF mouthrinse when it applies to human oral cavity.
Conclusion: Nano-HAp in 0.05% NaF mouthrinse has the potential to remineralize an early caries lesion.
키워드
구강양치액; 나노-수산화인회석; 불화나트륨; 재광화; 초기우식병소; pH 순환 모형
early caries Lesion; mouthrinse; NaF; nano-hydroxyapatite; pH cycling model; remineralization
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